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il 21  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec il 21
    Il 21, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+22/IL-22+Antibody%2C+anti-human%2C+REAfinity/pm41963361-193-39-42
    Average 96 stars, based on 6 article reviews
    il 21 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Bioprocessing:

    Article Title: Butyrate limits human natural killer cell effector function.
    Article Snippet: .. The following fluorochrome-conjugated monoclonal antibodies were used in this study: CD56 (REA196, NCAM 16.2), CD3 (SK7), CD71(M-A172); TRAIL (REA1113); NKG2D (1D11), NKp30 (P3015), NKp44 (REA1163), CD69 (FN50), CD161 (HP-3G10), Granzyme B (QA18A28), IFN-γ (4S.B3), IL-22 (2G12A41), TNF-α (MAb11) (Biolegend, Miltenyi Biotech or BD Bioscience). ..

    Article Title: Butyrate limits human natural killer cell effector function
    Article Snippet: .. The following fluorochrome-conjugated monoclonal antibodies were used in this study: CD56 (REA196, NCAM 16.2), CD3 (SK7), CD71(M-A172); TRAIL (REA1113); NKG2D (1D11), NKp30 (P30-15), NKp44 (REA1163), CD69 (FN50), CD161 (HP-3G10), Granzyme B (QA18A28), IFN-γ (4S.B3), IL-22 (2G12A41), TNF-α (MAb11) (Biolegend, Miltenyi Biotech or BD Bioscience). ..

    Ex Vivo:

    Article Title: Th22 cells are efficiently recruited in the gut by CCL28 as an alternative to CCL20 but do not compensate for the loss of Th17 cells in treated HIV-1-infected individuals.
    Article Snippet: This resulted in monolayers of differentiated epithelial cells expressing cytokeratin (CK)-18 and CK-20, EpCAM, and tight junctions revealed by continuous ZO-1 staining, as already published.28 The integrity of the epithelium was checked by measuring the transepithelial electrical resistance between the apical and basolateral sides of the monolayer (mean: 450Ω/cm2, as assessed using Millicell ERS-2, Millipore). .. Ex vivo stimulation of primary IEC by cytokines Primary IEC cultured on transwell inserts were stimulated for 15 h with 0.5, 5, or 50 ng/mL of human IL-17A, IL-17F, IL-22, TNF-α, and IL-1β (Miltenyi) added into the bottom chamber. .. Th17 and Th22 cell sorting and coculture with primary IEC Th17 and Th22 cells were sorted by flow cytometry on a FACSARIA II (BD Biosciences) from PBMCs of uninfected donors.

    Article Title: Glucocorticoid-induced leucine zipper (GILZ) inhibits B cell activation in systemic lupus erythematosus.
    Article Snippet: Centre for Inflammatory Diseases, School of Clinical Sciences at Monash Health, Monash University, Clayton, Melbourne, Australia Department of Human Genetics, Leiden Genome Technology Center, Leiden University Medical Center, Leiden, The Netherlands Department of Microbiology and Immunology, The Doherty Institute at The University of Melbourne, Parkville, Victoria, Australia Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia Department of Experimental Medicine, University of Melbourne, Parkville, Victoria, Australia

    Cell Culture:

    Article Title: Th22 cells are efficiently recruited in the gut by CCL28 as an alternative to CCL20 but do not compensate for the loss of Th17 cells in treated HIV-1-infected individuals.
    Article Snippet: This resulted in monolayers of differentiated epithelial cells expressing cytokeratin (CK)-18 and CK-20, EpCAM, and tight junctions revealed by continuous ZO-1 staining, as already published.28 The integrity of the epithelium was checked by measuring the transepithelial electrical resistance between the apical and basolateral sides of the monolayer (mean: 450Ω/cm2, as assessed using Millicell ERS-2, Millipore). .. Ex vivo stimulation of primary IEC by cytokines Primary IEC cultured on transwell inserts were stimulated for 15 h with 0.5, 5, or 50 ng/mL of human IL-17A, IL-17F, IL-22, TNF-α, and IL-1β (Miltenyi) added into the bottom chamber. .. Th17 and Th22 cell sorting and coculture with primary IEC Th17 and Th22 cells were sorted by flow cytometry on a FACSARIA II (BD Biosciences) from PBMCs of uninfected donors.

    Article Title: Circulating ILC precursors expressing CD62L exhibit a type 2 signature distinctly decreased in psoriatic patients
    Article Snippet: .. To evaluate IL‐22 and IL‐13 production by both CD62L neg and CD62L + ILCp, cells were sorted from blood of HDs according to CD62L expression and cultured for 7 days with human stromal cells [ ] in the presence of recombinant IL‐1β/IL‐23 (50 ng/mL, Miltenyi Biotec, each) or IL‐25/IL‐33 (50 ng/mL, Miltenyi Biotec, each). .. After 7 days, cells were stimulated with PMA (10 ng/mL) plus ionomycin (500 ng/mL) for 3 h in the presence of Monensin (2 μmol/L; Sigma–Aldrich) and Brefeldin (10 μg/ mL; Sigma–Aldrich) and cytokine production was assessed by intracellular staining.

    Expressing:

    Article Title: Circulating ILC precursors expressing CD62L exhibit a type 2 signature distinctly decreased in psoriatic patients
    Article Snippet: .. To evaluate IL‐22 and IL‐13 production by both CD62L neg and CD62L + ILCp, cells were sorted from blood of HDs according to CD62L expression and cultured for 7 days with human stromal cells [ ] in the presence of recombinant IL‐1β/IL‐23 (50 ng/mL, Miltenyi Biotec, each) or IL‐25/IL‐33 (50 ng/mL, Miltenyi Biotec, each). .. After 7 days, cells were stimulated with PMA (10 ng/mL) plus ionomycin (500 ng/mL) for 3 h in the presence of Monensin (2 μmol/L; Sigma–Aldrich) and Brefeldin (10 μg/ mL; Sigma–Aldrich) and cytokine production was assessed by intracellular staining.

    Recombinant:

    Article Title: Circulating ILC precursors expressing CD62L exhibit a type 2 signature distinctly decreased in psoriatic patients
    Article Snippet: .. To evaluate IL‐22 and IL‐13 production by both CD62L neg and CD62L + ILCp, cells were sorted from blood of HDs according to CD62L expression and cultured for 7 days with human stromal cells [ ] in the presence of recombinant IL‐1β/IL‐23 (50 ng/mL, Miltenyi Biotec, each) or IL‐25/IL‐33 (50 ng/mL, Miltenyi Biotec, each). .. After 7 days, cells were stimulated with PMA (10 ng/mL) plus ionomycin (500 ng/mL) for 3 h in the presence of Monensin (2 μmol/L; Sigma–Aldrich) and Brefeldin (10 μg/ mL; Sigma–Aldrich) and cytokine production was assessed by intracellular staining.

    Incubation:

    Article Title: Glucocorticoid-induced leucine zipper (GILZ) inhibits B cell activation in systemic lupus erythematosus.
    Article Snippet: Centre for Inflammatory Diseases, School of Clinical Sciences at Monash Health, Monash University, Clayton, Melbourne, Australia Department of Human Genetics, Leiden Genome Technology Center, Leiden University Medical Center, Leiden, The Netherlands Department of Microbiology and Immunology, The Doherty Institute at The University of Melbourne, Parkville, Victoria, Australia Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia Department of Experimental Medicine, University of Melbourne, Parkville, Victoria, Australia

    Staining:

    Article Title: Glucocorticoid-induced leucine zipper (GILZ) inhibits B cell activation in systemic lupus erythematosus.
    Article Snippet: Centre for Inflammatory Diseases, School of Clinical Sciences at Monash Health, Monash University, Clayton, Melbourne, Australia Department of Human Genetics, Leiden Genome Technology Center, Leiden University Medical Center, Leiden, The Netherlands Department of Microbiology and Immunology, The Doherty Institute at The University of Melbourne, Parkville, Victoria, Australia Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia Department of Experimental Medicine, University of Melbourne, Parkville, Victoria, Australia



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    Journal: Bioactive Materials

    Article Title: Dynamic feedback BacGuard anchors microbial metabolism to host symbiosis in real-time ulcerative colitis therapy

    doi: 10.1016/j.bioactmat.2026.05.060

    Figure Lengend Snippet: BacGuard promotes colon tissue repairment. a) Scheme of microbiota-dependent epithelial repair mechanism orchestrated by BacGuard. b) Short-chain fatty acid (SCFA) profile alterations. n = 6. c) BacGuard-induced probiotic proliferation and d) quantitative results. n = 3. e) Immunofluorescence analysis of ILC3 (ROR γt + CD3 − cells) in colon tissue. f) Flow cytometric analysis of lamina propria lymphocytes (ROR γt + ). n = 3. g) Concentration of IL-22 in MNK-3 cells. n = 3. h) Representative PAS-staining (upper panel) and MUC-2 immunohistochemistry (lower panel) images of colon tissues. n = 5. ns, not significant; ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001.

    Article Snippet: For the detection of IL-22 and LPS concentrations, the Mouse IL-22 Precoated ELISA Kit (DAKEWE, China) and Mouse LPS ELISA Kit (JONLNBIO, China) were employed correspondingly.

    Techniques: Immunofluorescence, Concentration Assay, Staining, Immunohistochemistry